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KMID : 1094720080130020150
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 2 p.150 ~ p.157
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KIOM-4 protects RINm5F pancreatic β-Cells against streptozotocin induced oxidative stress in vitro
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Kang Kyoung-Ah
Kim Jin-Sook
Hyun Jin-Won
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Abstract
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The effect of KIOM-4, a combination of four plant extracts was assessed on streptozotocin (STZ) treated rat insulinoma (RIN5mF) cells in vitro. KIOM-4 scavenged the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS) induced by STZ. KIOM-4 prevented the STZ-induced DNA damage, which is detected using comet assay, Western blot and lipid peroxidation assays. KIOM-4 inhibited the STZ induced apoptosis, therefore protecting from cell death. Additionally, KIOM-4 induced the activation of catalase and extracellular regulated kinase (ERK). These results suggest that KIOM-4 protects RINm5F cells via radical scavenging activity, the activation of catalase and ERK against STZ induced oxidative stress.
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KEYWORD
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oxidative stress, reactive oxygen species, KIOM-4, RIN5mF cells
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